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KMID : 0615720140310040033
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Jouranl of Korean Acupuncture and Moxibustion society
2014 Volume.31 No. 4 p.33 ~ p.43
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The Protective Effects of Acupuncture on the Liver in the Oxidative Stress Caused by Cadmium
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Shin Hwa-Young
Lee Hyun-Jong
Lim Seong-Chul
Lee Yun-Kyu
Lee Bong-Hyo
Jung Tae-Young
Kim Jae-Soo
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Abstract
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This study was performed to investigate the protective effects of acupuncture on the liver in the oxidative stress caused by cadmium accumulation.
Sprague-Dawley male(150 ± 30 g) rats were stabilized for a week and divided into 5 groups which is normal, control, LR3 acupuncture, BL23 acupuncture and sham acupuncture group. For three days experimental groups were received oral doses of cadmium 2 mg/kg twice a day. Acupuncture was given bilaterally at each point 10 times for two weeks. The depth of stimulation was 1 mm at right angles and torsion of acupuncture was produced 2 times per second for 1 minutes. The liver was shipped off and taken weight at the last day of two weeks, and hepatic functions was confirmed through alanine transaminase(ALT) and aspartate aminotransferase(AST). We measured reactive oxygen species of serum, liver and kidney, and compared expression levels of superoxide dismutase(SOD), catalase, glutathione peroxidase(Gpx), nuclear factor erythroid derived 2-related factor 2(Nrf-2), heme oxygenase-1(HO-1), nuclear factor-κB (NF-κB), cyclooxygenase-2(COX-2), inducible nitric oxide synthase(iNOS), Bax and Cytochrome c. BL23 acupuncture group significantly increased liver weight and decreased ALT compared to control group. For the oxidative stress, LR3 acupuncture group significantly reduced reactive oxygen species, and BL23 acupuncture group significantly reduced reactive oxygen species and inflammation-related protein compared to control group. But LR3 acupuncture group and BL23 acupuncture group didn’t significantly reduce apoptosis-related protein.
Therefore LR3 and BL23 acupuncture showed the effects of antioxidant and anti-inflammatory, especially BL23 acupuncture was more effective than LR3 acupuncture on the protection of liver in the oxidative stress.
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KEYWORD
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Acupuncture, Cadmium, Oxidative stress, LR3, BL23.
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